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secondary antibody solution  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology secondary antibody solution
    Secondary Antibody Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 9587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+solution/IgG+Antibody/pmc12641226-90-67-74
    Average 96 stars, based on 9587 article reviews
    secondary antibody solution - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Gut microbial metabolite TMAO impairs cognitive function and induces hippocampal synaptic plasticity decline through modulation of GSK-3β activity
    Article Snippet: The separated proteins were transferred onto a PVDF membrane (Millipore) and incubated overnight at 4 °C based on the following primary antibodies: GSK-3β (1:1000, Cell Signaling), phospho-Ser9-GSK-3β (1:1000, Cell Signaling), phospho-Tyr216-GSK-3β (1:1000, Abcam), α-tubulin (1:2000, Invitrogen), PSD95 (1:1000, Abcam), and Synapsin I (1:1000, Abcam); GluA1 (1:1000, Abcam). .. After washing, the membranes were incubated using a secondary antibody solution (goat anti-mouse or goat anti-rabbit IgG-HRP, 1:5000, Santa Cruz) at room temperature for 2 h, then detected with the enhanced chemiluminescence (ECL) method. ..

    Article Title: Diosgenin Attenuates Myocardial Cell Apoptosis Triggered by Oxidative Stress through Estrogen Receptor to Activate the PI3K/Akt and ERK Axes
    Article Snippet: Cardiovascular diseases in post-menopausal women are on a rise.. Oxidative stress is the main contributing factor to the etiology and pathogenesis of cardiovascular diseases.. Diosgenin, a member of steroidal sapogenin, is structurally similar to estrogen and has been shown to have antioxidant effects.

    Article Title: Tea Tree Oil Improves Energy Metabolism, Non-Specific Immunity, and Microbiota Diversity via the Intestine-Hepatopancreas Axis in Macrobrachium rosenbergii under Low Fish Meal Diet Administration.
    Article Snippet: .. After equilibration at room temperature for 1 h, the membranes were washed three times for 10 min each and incubated with a secondary antibody solution (goat anti-rabbit horseradish peroxidase conjugate, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at RT. .. An electrochemiluminescence (ECL) kit (Beyotime, P0018FM, China) was used to visualize the immune complexes, and ImageJ software 1.52a was used for quantification.

    Article Title: Gut microbial metabolite TMAO impairs cognitive function and induces hippocampal synaptic plasticity decline through modulation of GSK-3β activity.
    Article Snippet: The separated proteins were transferred onto a PVDF membrane (Millipore) and incubated overnight at 4 °C based on the following primary antibodies: GSK-3β (1:1000, Cell Signaling), phospho-Ser9-GSK-3β (1:1000, Cell Signaling), phospho-Tyr216-GSK-3β (1:1000, Abcam), α-tubulin (1:2000, Invitrogen), PSD95 (1:1000, Abcam), and Synapsin I (1:1000, Abcam); GluA1 (1:1000, Abcam). .. After washing, the membranes were incubated using a secondary antibody solution (goat anti-mouse or goat anti-rabbit IgG-HRP, 1:5000, Santa Cruz) at room temperature for 2 h, then detected with the enhanced chemiluminescence (ECL) method. ..

    Article Title: Forced Activation of β-Catenin Signaling Supports the Transformation of hTERT-Immortalized Human Fetal Hepatocytes
    Article Snippet: Primary antibody solution detecting b-catenin (1:200; Santa Cruz Biotechnology) was incubated at 4 C overnight. .. Incubation with species-specific secondary antibody solution (1:1,000; Santa Cruz Biotechnology) conjugated with horseradish peroxidase was carried out for 45 minutes at room temperature. .. Detection was achieved with the ECL Western Blotting Analysis System (Amersham Biosciences).

    Saline:

    Article Title: Diosgenin Attenuates Myocardial Cell Apoptosis Triggered by Oxidative Stress through Estrogen Receptor to Activate the PI3K/Akt and ERK Axes
    Article Snippet: Cardiovascular diseases in post-menopausal women are on a rise.. Oxidative stress is the main contributing factor to the etiology and pathogenesis of cardiovascular diseases.. Diosgenin, a member of steroidal sapogenin, is structurally similar to estrogen and has been shown to have antioxidant effects.

    Blocking Assay:

    Article Title: Kainic acid status epilepticus - induced changes in the hippocampus and hypothalamus alter the effects of exogenous Orexin-A in the hippocampus
    Article Snippet: For detection of Orexinergic cells in hypothalamus Goat anti orexin A antibodies (Santa Crus biotechnology) were used. .. After blocking of endogenous peroxidase with 3 % hydrogen peroxide, preincubation with 2 % BSA (2 %BSA (Sigma A9647) + 2.5 % normal goat serum (Sigma Aldrich G9023) + 0.5 % Triton X-100 (Sigma Aldrich T9284) was performed at 37°C for 30 min. Primary antibody (Goat anti orexin A, Santa Crus biotechnology) solution (dilution 1:1000) was added for 24 h at + 4 0 C temperature, then secondary antibody solution (Swine anti goat IgG Santa Crus biotechnology) (dilution 1:400) for 2 h at room temperature. ..



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